Part of the global effort to discover new antibiotics involves inventing new techniques to analyze the ones we already have. The idea is that the more we learn about how antimicrobials work at the molecular level, the easier it will be to find or synthesize novel ones.

One way to learn about how antibiotics work is to visualize their accumulation within bacterial cells. But this is no easy feat. Several imaging techniques already exist, such as monitoring fluorescence or tracking molecules using radioactive labels, but these methods suffer from various drawbacks. So, a team of researchers from Penn State University and the pharmaceutical and biotechnology giant Novartis set about inventing a new technique.